The above estimates are based on the numbers provided below, plus a little bit of rounding up for incidentals and labor and such. The cost of making 24 (meta)genomic libraries, using the TruSeq kit (preferred for high-input samples) and pooling them for a single MiSeq PE250 run is approximately $2600.The cost of making 24 (meta)genomic libraries, using the Nextera XT kit (required for low-input samples) and pooling them for a single MiSeq PE250 run is approximately $2100. ![]() This includes the cost for DNA extraction, PCR primers and reagents, and the sequencing. The cost of making 100 amplicon libraries and pooling them for a single MiSeq PE250 run is approximately $1700.
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June 2023
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